The decision making process and the step forward through the implementation of the environmental value inside organizations is relatively recent. Nowadays the ISO 14001 Standard is the most common certificate used by organizations aiming the responsibility concerning nature and its environment. The fact is that this kind of implementation process and the maintenance requires a sort of changes, new practices and inversions in its interior and moreover in its people. Generally, the Environmental Management Systems (EMS) are based in a series of environmental behaviors that reflect on the workers routine and perhaps in their environmental behavior outside the company. It requires a sort of changes in its process, structures and human resources and also attempt to adopt and identify with the sustainability values, explicit in the establishment of the environmental policy (Pol, 2002). Furthermore, as Pol (2003) says, Environmental Management is the responsible of the incorporation of the values of the sustainable development in the social organization and the corporative goals of the company or the public administration and its role in the development process of interest and concern about the environment is quite significant. Ecological behavior is defined as actions that contribute towards environmental preservation and/or conservations, including recycling, energy and water conservation, political activism, consumerism and so forth (Kaiser & Fuhrer, 2003). Stern (2000) refers, that the environmental behavior should be defined by its impact: the extent to which it changes, the availability of materials or the materials or energy from the environment or alters structure and dynamics of ecosystems or the biosphere itself. The aim of the present work is to analyze the effect of being used to EMS in the environmental behavior of Brazilian workers. Data collection took place in 2008, in Brazil, having workers from certified organization (n=232), non certified organization (n=153) and non certified with environmental policy (n=169). The analysis points that the there are significant differences between environmental behavior of the three groups. Also was possible to identify, with the contrast test Scheffé, that there were significant differences between the means of the certified group and the others two groups. The results can be used as starting point to emphasize the important role of the organizations and the institutions in the process of development of environmental concern in their workers. It is a fact that there is a lack of studies that examine the relationship between these variables in Brazil in a workers sample, so, this research intent to contribute with this continuum construction of this line of investigation and future practical interventions in that country.