Although levels of green space have been associated with positive health benefits, including stress reduction, very few studies have investigated these associations using stress biomarkers with participants in everyday, residential settings. This paper follows an earlier, exploratory study (Ward Thompson et al., (under review 2011)that showed significant relationships between self-reported stress, the diurnal pattern of cortisol secretion, and quantity of green space in the residential environment using a small sample from a deprived UK city (Dundee) (n=25). This study replicates the study design in a larger sample (n=106), comprising multiple measurement of salivary cortisol over two days in men and women not in work, aged 35-55, in the same city context. The sensitivity of measures of green space in predicting patterns of cortisol concentrations in participants was tested using both a continuous measure of percentage green space in the neighbourhood and a binary category of green space split at a regression discriminator of 43%. Results from linear regression analyses show a significant and positive effect of higher green space levels on self-reported stress and diurnal slope patterns of salivary cortisol (3, 6 and 9 hours post awakening). Main effects of gender were found on self-reported stress, with stress being higher for women. Significant interaction effects between gender and percentage green space were found on mean cortisol concentrations, showing a positive effect of increasing green space on cortisol levels for women, but not for men. This study confirms and extends findings from our earlier study showing how - in deprived city areas – higher levels of green space close to home are associated with better health and how these effects may be particularly pertinent to women and/or those in poorer mental health.